Dynamic monitoring of osteoarthritis-associated cellular and molecular changes using CMOS MEA technology

Osteoarthritis (OA) is a progressive joint disease marked by the degeneration of articular cartilage, subchondral bone remodelling, and inflammation, ultimately leading to significant pain and functional impairment. Despite its widespread prevalence and profound impact on quality of life, the underlying cellular and molecular mechanisms remain poorly understood. Recent advancements in technology, particularly in microelectrode array (MEA) systems, offer innovative tools to investigate the intricate cellular dynamics and molecular signalling pathways involved in OA pathogenesis. This research aims to employ complementary metal-oxide-semiconductor (CMOS) MEA technology to explore the cellular and molecular alterations triggered by OA-relevant stimuli in critical cell types, including chondrocytes, synoviocytes, and osteoblasts.
 
The proposed project will apply various OA-inducing stimuli, such as pro-inflammatory cytokines (e.g., IL-1β, TNF-α), to cultured OA-relevant cells. By utilizing CMOS MEA technology, we will monitor impedance changes, which will provide insights into alterations in cell-cell and cell-matrix adhesion as well as cell morphology. Additionally, we will conduct immunocytochemistry assays to correlate impedance values with key molecular changes. The high-resolution temporal and spatial capabilities of CMOS MEAs, combined with standard molecular assays, will allow us to elucidate how OA stimuli influence cellular communication and function within these critical cell types.
 
In summary, harnessing CMOS MEA technology to investigate the cellular and molecular changes in OA-relevant cell types represents a promising approach to unravelling the complexities of osteoarthritis. Participants in this project will have the opportunity to learn and practice a variety of biological techniques, including cell culture, immunocytochemistry, MEA-based impedance monitoring, and both epifluorescence and confocal microscopy. Participants with prior experience in cell culture and electrophysiology would be given priority.
 

Type of Project: Internship 

Duration: 6 months - 1 year 

Master's degree: Master of Science; Master of Bioengineering 

Master program: Biomedical engineering; Bioscience Engineering; Nanoscience & Nanotechnology; Other 

For more information or application, please contact Neha Deshpande (neha.deshpande@imec.be) and Johanna Bolander (johanna.bolander@imec.be).

Only for self-supporting students.