Enhancing CAR-T cell therapy using microfluidic surface modifications for label-free cell characterization

Cell therapy is an innovative approach in modern medicine, offering personalized treatments for a variety of diseases, with CAR-T therapy serving as a notable example in the field of cancer immunotherapy. A critical step in this process is the accurate characterization and classification of immune cells in their natural state. Conventional techniques like flow cytometry rely on fluorescence labeling, which alters the natural environment of cells and complicates sample preparation. These limitations make it less suitable for use in CAR-T cell production. To address this issue, imec has developed a label-free microfluidic platform that uses antibody-functionalized surfaces to classify cells. This method maintains cell integrity and is designed to be directly integrated into the production workflow for therapeutic applications.
 
Despite its potential, the platform requires further development. The current surface functionalization protocols need to be optimized to improve sensitivity and selectivity in cell classification. Additionally, the ability to profile multiple cell markers simultaneously needs to be incorporated to better identify specific subsets of cells. Addressing these challenges will enhance the platform’s accuracy and usability in CAR-T cell manufacturing.
 
This thesis aims to enhance surface modification protocols to improve the performance of the microfluidic devices for cell classification based on antibody interactions. To this end, the student will design and fabricate new microfluidic flow channels. Using statistical experimental design, the surface chemistry and antibody functionalization protocols will be optimized to improve classification performance. Hereto, the cell interaction dynamics will be assessed using imec’s image-based sensing technology. The project will also work toward integrating multiple antibody layers into a single device for simultaneous analysis of various cell markers.
 
We are looking for an enthusiastic master thesis or internship candidate with a background in (bio)chemistry, nanotechnology, and biomedical engineering. Under supervision of a multidisciplinary team, the student is able to perform independent, hands-on work in a laboratory environment. Additionally, the student is proficient with data analysis software, statistical design and can communicate scientific results efficiently. The results of this work will contribute to the advancement of cell characterization with the potential to further improve CAR-T cell therapy processes.
 

Type of Project: Thesis; Internship 

Master's degree: Master of Bioengineering; Master of Science 

Master program: Bioscience Engineering 

Duration: 9-12 months 

Supervisor: Philippe Vereecken (Bioscience, Nano) 

For more information or application, please contact the supervising scientists Philippe Vereecken (philippe.vereecken@imec.be) and Thijs Roebroek (thijs.roebroek@imec.be).

Imec allowance will be provided for students studying at a non-Belgian university.